We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A system for stable expression of short interfering RNAs in mammalian cells.
- Authors
Brummelkamp, Thijn R; Bernards, René; Agami, Reuven
- Abstract
Mammalian genetic approaches to study gene function have been hampered by the lack of tools to generate stable loss-of-function phenotypes efficiently. We report here a new vector system, named pSUPER, which directs the synthesis of small interfering RNAs (siRNAs) in mammalian cells. We show that siRNA expression mediated by this vector causes efficient and specific down-regulation of gene expression, resulting in functional inactivation of the targeted genes. Stable expression of siRNAs using this vector mediates persistent suppression of gene expression, allowing the analysis of loss-of-function phenotypes that develop over longer periods of time. Therefore, the pSUPER vector constitutes a new and powerful system to analyze gene function in a variety of mammalian cell types.
- Publication
Science (New York, N.Y.), 2002, Vol 296, Issue 5567, p550
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1068999