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- Title
Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.
- Authors
Winston, William M; Molodowitch, Christina; Hunter, Craig P
- Abstract
Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference-deficient (sid) loci required to spread gene-silencing information between tissues but not to initiate or maintain an RNAi response. One of these loci, sid-1, encodes a conserved protein with predicted transmembrane domains. SID-1 is expressed in cells sensitive to RNAi, is localized to the cell periphery, and is required cell-autonomously for systemic RNAi.
- Publication
Science (New York, N.Y.), 2002, Vol 295, Issue 5564, p2456
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1068836