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- Title
Coordination of PIC assembly and chromatin remodeling during differentiation-induced gene activation.
- Authors
Soutoglou, Evi; Talianidis, Iannis
- Abstract
We analyzed the ordered recruitment of factors to the human alpha1 antitrypsin promoter around the initial activation of the gene during enterocyte differentiation. We found that a complete preinitiation complex, including phosphorylated RNA pol II, was assembled at the promoter long before transcriptional activation. The histone acetyltransferases CBP and P/CAF were recruited subsequently, but local histone hyperacetylation was delayed. After transient recruitment of the human Brahma homolog hBrm, remodeling of the neighboring nucleosome coincided with transcription initiation. The results suggest that, at this promoter, chromatin reconfiguration is a defining step of the initiation process, acting after the assembly of the Pol II machinery.
- Publication
Science (New York, N.Y.), 2002, Vol 295, Issue 5561, p1901
- ISSN
1095-9203
- Publication type
Journal Article
- DOI
10.1126/science.1068356