We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Role of cell-specific SpoIIIE assembly in polarity of DNA transfer.
- Authors
Sharp, Marc D; Pogliano, Kit
- Abstract
SpoIIIE mediates postseptational chromosome partitioning in Bacillus subtilis, but the mechanism controlling the direction of DNA transfer remains obscure. Here, we demonstrated that SpoIIIE acts as a DNA exporter: When SpoIIIE was synthesized in the larger of the two cells necessary for sporulation, the mother cell, DNA was translocated into the smaller forespore; however, when it was synthesized in the forespore, DNA was translocated into the mother cell. Furthermore, the DNA-tracking domain of SpoIIIE inhibited SpoIIIE complex assembly in the forespore. Thus, during sporulation, chromosome partitioning is controlled by the preferential assembly of SpoIIIE in one daughter cell.
- Publication
Science (New York, N.Y.), 2002, Vol 295, Issue 5552, p137
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1066274