We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Defective antigen processing in GILT-free mice.
- Authors
Maric, M; Arunachalam, B; Phan, U T; Dong, C; Garrett, W S; Cannon, K S; Alfonso, C; Karlsson, L; Flavell, R A; Cresswell, P
- Abstract
Processing of proteins for major histocompatibility complex (MHC) class II-restricted presentation to CD4-positive T lymphocytes occurs after they are internalized by antigen-presenting cells (APCs). Antigenic proteins frequently contain disulfide bonds, and their reduction in the endocytic pathway facilitates processing. In humans, a gamma interferon-inducible lysosomal thiol reductase (GILT) is constitutively present in late endocytic compartments of APCs. Here, we identified the mouse homolog of GILT and generated a GILT knockout mouse. GILT facilitated the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. The response to hen egg lysozyme, a model antigen with a compact structure containing four disulfide bonds, was examined in detail.
- Publication
Science (New York, N.Y.), 2001, Vol 294, Issue 5545, p1361
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1065500