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- Title
A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA.
- Authors
Hutvágner, G; McLachlan, J; Pasquinelli, A E; Bálint, E; Tuschl, T; Zamore, P D
- Abstract
The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.
- Publication
Science (New York, N.Y.), 2001, Vol 293, Issue 5531, p834
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1062961