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- Title
Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.
- Authors
Lykke-Andersen, J; Shu, M D; Steitz, J A
- Abstract
In mammalian cells, splice junctions play a dual role in mRNA quality control: They mediate selective nuclear export of mature mRNA and they serve as a mark for mRNA surveillance, which subjects aberrant mRNAs with premature termination codons to nonsense-mediated decay (NMD). Here, we demonstrate that the protein RNPS1, a component of the postsplicing complex that is deposited 5' to exon-exon junctions, interacts with the evolutionarily conserved human Upf complex, a central component of NMD. Significantly, RNPS1 triggers NMD when tethered to the 3' untranslated region of beta-globin mRNA, demonstrating its role as a subunit of the postsplicing complex directly involved in mRNA surveillance.
- Publication
Science (New York, N.Y.), 2001, Vol 293, Issue 5536, p1836
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1062786