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- Title
Global analysis of protein activities using proteome chips.
- Authors
Zhu, H; Bilgin, M; Bangham, R; Hall, D; Casamayor, A; Bertone, P; Lan, N; Jansen, R; Bidlingmaier, S; Houfek, T; Mitchell, T; Miller, P; Dean, R A; Gerstein, M; Snyder, M
- Abstract
To facilitate studies of the yeast proteome, we cloned 5800 open reading frames and overexpressed and purified their corresponding proteins. The proteins were printed onto slides at high spatial density to form a yeast proteome microarray and screened for their ability to interact with proteins and phospholipids. We identified many new calmodulin- and phospholipid-interacting proteins; a common potential binding motif was identified for many of the calmodulin-binding proteins. Thus, microarrays of an entire eukaryotic proteome can be prepared and screened for diverse biochemical activities. The microarrays can also be used to screen protein-drug interactions and to detect posttranslational modifications.
- Publication
Science (New York, N.Y.), 2001, Vol 293, Issue 5537, p2101
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1062191