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- Title
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
- Authors
Nakayama, J; Rice, J C; Strahl, B D; Allis, C D; Grewal, S I
- Abstract
The assembly of higher order chromatin structures has been linked to the covalent modifications of histone tails. We provide in vivo evidence that lysine 9 of histone H3 (H3 Lys9) is preferentially methylated by the Clr4 protein at heterochromatin-associated regions in fission yeast. Both the conserved chromo- and SET domains of Clr4 are required for H3 Lys9 methylation in vivo. Localization of Swi6, a homolog of Drosophila HP1, to heterochomatic regions is dependent on H3 Lys9 methylation. Moreover, an H3-specific deacetylase Clr3 and a beta-propeller domain protein Rik1 are required for H3 Lys9 methylation by Clr4 and Swi6 localization. These data define a conserved pathway wherein sequential histone modifications establish a "histone code" essential for the epigenetic inheritance of heterochromatin assembly.
- Publication
Science (New York, N.Y.), 2001, Vol 292, Issue 5514, p110
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1060118