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- Title
Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.
- Authors
Lyapina, S; Cope, G; Shevchenko, A; Serino, G; Tsuge, T; Zhou, C; Wolf, D A; Wei, N; Shevchenko, A; Deshaies, R J
- Abstract
SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how SCF complexes are regulated, we sought proteins that interact with the human SCF component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated with multiple cullins and promoted cleavage of the ubiquitin-like protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo and in vitro. Multiple NEDD8-modified proteins uniquely accumulated in CSN-deficient S. pombe cells. We propose that the broad spectrum of activities previously attributed to CSN subunits--including repression of photomorphogenesis, activation of JUN, and activation of p27 nuclear export--underscores the importance of dynamic cycles of NEDD8 attachment and removal in biological regulation.
- Publication
Science (New York, N.Y.), 2001, Vol 292, Issue 5520, p1382
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1059780