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- Title
TRP-PLIK, a bifunctional protein with kinase and ion channel activities.
- Authors
Runnels, L W; Yue, L; Clapham, D E
- Abstract
We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing alpha-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.
- Publication
Science (New York, N.Y.), 2001, Vol 291, Issue 5506, p1043
- ISSN
0036-8075
- Publication type
Journal Article
- DOI
10.1126/science.1058519