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- Title
Carcinogen treatment in mouse selectively expressing activated N-Ras<sup>Q61K</sup> in melanocytes recapitulates metastatic cutaneous melanoma development.
- Authors
Contassot, Emmanuel; Jankovic, Dragana; Schuler, Prisca; Preynat-Seauve, Olivier; Gehrke, Samuel; Kerl, Katrin; Beermann, Friedrich; French, Lars E.
- Abstract
Summary The incidence of melanoma has significantly increased, and a better understanding of its pathogenesis and development of new therapeutic strategies are urgently needed. Here, we describe a murine model of metastatic cutaneous melanoma using C57BL/6 mice expressing a mutated human N-Ras gene under the control of a tyrosinase promoter (TyrRas). These mice were topically exposed to 7,12- dimethylbenzanthracene (DMBA) for brief exposure periods. Cutaneous melanoma developed at the site of exposure on average by 19 weeks of age and in 80% of mice. Importantly, as in humans, melanoma development was associated with subsequent metastasis to tumor-draining lymph nodes. Critically, such metastatic behavior is transplantable, as intradermal inoculation of melanoma cells from TyrRas-DMBA mice into non-transgenic mice led to the growth of melanoma and, again, metastasis to skin-draining lymph nodes. This metastatic melanoma model closely mimics human pathology and should be a useful tool for studying melanoma pathogenesis and developing new therapies.
- Publication
Pigment Cell & Melanoma Research, 2012, Vol 25, Issue 2, p275
- ISSN
1755-1471
- Publication type
Academic Journal
- DOI
10.1111/j.1755-148X.2011.00944.x