Role of Neutrophils in Arginine-asymmetric Dimethylarginine Pathway Subsequent to Endotoxemia.

Nurten, Türközkan; Nihal, Şahin; Gonca, Ozan; Özgür, Akgül; Halil, Yaman; Filiz, Bircan; Barbaros, Balabanli

There is a striking correlation between nitric oxide (NO) production and myeloperoxidase (MPO) enzyme activity accession in various tissues after endotoxemia. Both arginine–NO and arginine-asymmetric dimethylarginine (ADMA) pathways have been recognized to play critical roles during infection and inflammation. We investigated whether there is a link between MPO-mediated nitrating pathway and arginine–ADMA pathway after endotoxemia in liver. All experiments were performed in two groups (control and endotoxemia) of 10 guinea pigs. In this study, 6 h after the administration of endotoxin at a dose of 4 mg/kg, MPO activity and ADMA, L-arginine, and 3-nitrotyrosine (3-NT, a stable product of peroxynitrite formation) levels were measured. Measurement of ADMA and L-arginine were accomplished by HPLC with fluorescent detector. 3-NT was quantified by HPLC with electrochemical detector. MPO activity was determined by spectrophotometric method. After administration of endotoxin, ADMA and L-arginine levels decreased, but 3-NT levels and MPO activity increased significantly. In conclusion, there is an inverse relationship between MPO-mediated tyrosine nitration and arginine–ADMA pathway in liver after endotoxemia.

NEUTROPHILS; ARGININE; ENDOTOXEMIA; ENDOTOXINS; SPECTROPHOTOMETRY; ELECTROCHEMICAL sensors

Annals of the New York Academy of Sciences, 2009, Vol 1173, pE55

0077-8923

Academic Journal

10.1111/j.1749-6632.2009.04951.x