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- Title
Generation and Characterization of C305, a Murine Neutralizing scFv Antibody That Can Inhibit BLyS Binding to Its Receptor BCMA.
- Authors
Mei-Yun Liu; Wei Han; Yan-Li Ding; Tian-Hong Zhou; Rui-Yang Tian; Sheng-Li Yang; Hui Liu; Yi Gong
- Abstract
B-lymphocyte stimulator (BLyS) is a member of the tumor necrosis factor (TNF) family and a key regulator of B cell response. Neutralizing single-chain fragment variable (scFv) antibody against BLyS binding to its receptor BCMA has the potential to play a prominent role in autoimmune disease therapy. A phage display scFv library constructed on pIII protein of M13 filamentous phage was screened using BLyS. After five rounds of panning, their binding activity was characterized by phage-ELISA. Nucleotide sequencing revealed that at least two different scFv gene fragments (C305 and D416) were obtained. The two different scFv gene fragments were expressed to obtain the soluble scFv antibodies, then the soluble scFv antibodies were characterized by means of competitive ELISA and in vitro neutralization assay. The results indicated that C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA. Edited by: Zu-Chuan ZHANG
- Publication
Acta Biochimica et Biophysica Sinica, 2005, Vol 37, Issue 6, p415
- ISSN
1672-9145
- Publication type
Academic Journal
- DOI
10.1111/j.1745-7270.2005.00059.x