We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion.
- Authors
Brunet, Stéphane; Zimmermann, Timo; Reynaud, Emmanuel G; Vernos, Isabelle; Karsenti, Eric; Pepperkok, Rainer
- Abstract
We present an in vitro system to analyze quantitatively the interactions of green fluorescent protein (GFP)-tagged recombinant proteins with microtubules. This method relies on photoconversion of GFP and time-lapse microscopy. Specific interactions can be detected and binding kinetics can be determined rapidly and accurately. This method provides an alternative to classical in vitro microtubule-binding assays to analyze microtubule-associated proteins binding to microtubules. It has the potential to be extended to study interactions of proteins or multi-protein complexes with different biopolymers like actin microfilaments or organelle membranes.
- Publication
Traffic (Copenhagen, Denmark), 2006, Vol 7, Issue 9, p1283
- ISSN
1398-9219
- Publication type
Journal Article
- DOI
10.1111/j.1600-0854.2006.00463.x