We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Shosaikoto increases calprotectin expression in human oral epithelial cells.
- Authors
Hiroshima, Y.; Bando, M.; Kataoka, M.; Shinohara, Y.; Herzberg, M. C.; Ross, K. F.; Inagaki, Y.; Nagata, T.; Kido, J.
- Abstract
Hiroshima Y, Bando M, Kataoka M, Shinohara Y, Herzberg MC, Ross KF, Inagaki Y, Nagata T, Kido J. Shosaikoto increases calprotectin expression in human oral epithelial cells. J Periodont Res 2009; doi: 10.1111/j.1600-0765.2009.01203.x. © 2009 John Wiley & Sons A/S Background and Objective: Oral epithelial cells help to prevent against bacterial infection in the oral cavity by producing antimicrobial peptides (AMPs). A broad-spectrum AMP, calprotectin (a complex of S100A8 and S100A9 proteins), is expressed by oral epithelial cells and is up-regulated by interleukin-1α (IL-1α). Shosaikoto (SST) is a traditional Japanese herbal medicine that has immunomodulatory effects and is reported to enhance the levels of IL-1α in epithelial cells. The purpose of this study was to investigate the effect of SST on the expression of calprotectin and other AMPs through the regulation of IL-1α in oral epithelial cells. Material and Methods: Human oral epithelial cells (TR146) were cultured with SST (at concentrations ranging from 10 to 250 μg/mL) in the presence or absence of anti-IL-1α or IL-1 receptor antagonist. The expression of S100A8- and S100A9-specific mRNAs was examined by northern blotting. Calprotectin expression and IL-1α secretion were investigated by immunofluorescent staining or ELISA. The expression of other AMPs and IL-1α was analyzed by RT-PCR and by quantitative real-time PCR. Results: Shosaikoto (25 μg/mL) significantly increased the expression of S100A8- and S100A9-specific mRNAs and calprotectin protein. Shosaikoto increased S100A7 expression, but had no effect on the expression of other AMPs. The expression of IL-1α-specific mRNA and its protein were slightly increased by SST. A neutralizing antibody against IL-1α or IL-1 receptor antagonist inhibited SST up-regulated S100A8/S100A9 mRNA expression. Conclusion: These results suggest that SST increases the expression of calprotectin and S100A7 in oral epithelial cells. In response to SST, up-regulation of calprotectin may be partially induced via IL-1α.
- Publication
Journal of Periodontal Research, 2010, Vol 45, Issue 1, p79
- ISSN
0022-3484
- Publication type
Academic Journal
- DOI
10.1111/j.1600-0765.2009.01203.x