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- Title
p63 directly induces expression of Alox12, a regulator of epidermal barrier formation.
- Authors
Soeun Kim; Choi, Irene F.; Quante, Jessica R.; Lei Zhang; Roop, Dennis R.; Koster, Maranke I.
- Abstract
Epidermal development and differentiation are tightly controlled processes that culminate in the formation of the epidermal barrier. A critical regulator of different stages of epidermal development and differentiation is the transcription factor p63. More specifically, we previously demonstrated elsewhere that p63 is required for both the commitment to stratification and the commitment to terminal differentiation. We now demonstrate that ΔNp63α, the predominantly expressed p63 isoform in postnatal epidermis, also plays a role in the final stages of epidermal differentiation, namely the formation of the epidermal barrier. We found that ΔNp63α contributes to epidermal barrier formation by directly inducing expression of ALOX12, a lipoxygenase which contributes to epidermal barrier function. Our data demonstrate that ΔNp63α directly interacts with the promoter of Alox12 in the developing epidermis. Furthermore, we found that the induction of Alox12 expression by ΔNp63α depends on intact p63 binding sites in the Alox12 promoter. Finally, we found that ΔNp63α can induce Alox12 expression only in differentiating keratinocytes, consistent with the role of ALOX12 in epidermal barrier formation.
- Publication
Experimental Dermatology, 2009, Vol 18, Issue 12, p1016
- ISSN
0906-6705
- Publication type
Academic Journal
- DOI
10.1111/j.1600-0625.2009.00894.x