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- Title
Increased population of CD4<sup>+</sup>CD25<sup>high</sup> regulatory T cells with their higher apoptotic and proliferating status in peripheral blood of acute myeloid leukemia patients.
- Authors
Xingbing Wang; Jine Zheng; Liu, Jun; Junxia Yao; Yanli He; Xiaoqing Li; Jingming Yu; Jing Yang; Zhongping Liu; Shiang Huang
- Abstract
Purpose: Regulatory T cells (T-reg) that control harmful autoimmune T cells in the periphery may also suppress the immune response against cancer. In this study we investigated the possible involvement of CD4+CD25high T-reg in the immune impairment of patients with acute myeloid leukemia (AML). Experimental design: The frequencies and phenotypes of CD4+CD25high T cells in the peripheral blood of AML patients were determined by flow cytometry. To assess the functional activity of CD4+CD25high T cells, CD4+CD25high, and CD4+CD25− T cells were sorted from peripheral blood mononuclear cells with FACS Vantage. The immunoregulatory properties of CD4+CD25high and CD4+CD25− T cells were characterized by proliferation assays and cytokine production assays. In addition, the frequency of apoptotic and proliferating cells in CD4+CD25high T cells were respectively evaluated by 7AAD and ki67 binding cells using flow cytometry. Results: Compared with healthy controls, AML patients had a higher proportion of CD4+CD25high T cells in peripheral blood. These cells were CD45-RA(−), CD69(−), CD45-RO(+), CD95(+), and intercellular CTLA-4(+), and secreted low levels of TNF- α and IL-10, but no IL-2, IL-4, IL-5, and IFN- γ. They inhibited the proliferation and cytokine production (IL-2, IFN- γ) of CD4+CD25− T cells, but improved IL-10 production under the co-culture of both subsets with stimulation, thus behaving as T-reg. Notably, CD4+CD25high T cells in AML patients presented significantly higher apoptosis and proliferation than that of healthy individuals. Conclusions: The frequency of CD4+CD25high T-reg in peripheral blood in AML patients is significantly higher when compared with healthy individuals, likely due to the increasing proliferation of CD4+CD25high T cells.
- Publication
European Journal of Haematology, 2005, Vol 75, Issue 6, p468
- ISSN
0902-4441
- Publication type
Academic Journal
- DOI
10.1111/j.1600-0609.2005.00537.x