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- Title
Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR.
- Authors
Park, Si Hong; Kim, Hyun Joong; Cho, Woo Hee; Kim, Jae Hwan; Oh, Mi Hwa; Kim, Sung Hun; Lee, Bok Kwon; Ricke, Steven C; Kim, Hae Yeong
- Abstract
This study was designed to develop a multiplex PCR method with five specific primer pairs for the detection of Salmonella spp., Salmonella subspecies I, Salmonella enterica serovars Typhimurium, Typhi and Enteritidis. A multiplex PCR was constructed with five primer pairs for the detection of Salmonella and pathogenic Salmonella serovars, including a specific primer pair for Salmonella Typhi, based on the sequence comparison between genomic DNA sequences of 12 Salmonella strains. Each primer pair was specifically targeted to Salmonella spp., Salmonella subspecies I, Salmonella Typhimurium, Typhi and Enteritidis. This multiplex PCR was evaluated with various DNAs of Salmonella serovars that yielded high specificity for amplifying the expected PCR products of Salmonella serovars. Using this primer pair, a set of multiplex PCR was performed for the rapid identification of salmonellae and major pathogenic Salmonella serovars. Although this multiplex PCR method will need to be evaluated for a wide range of Salmonella serovars among multilaboratories, it should be useful for identifying clinically significant strains of Salmonella serovars rapidly and accurately without the need for serological testing.
- Publication
FEMS microbiology letters, 2009, Vol 301, Issue 1, p137
- ISSN
1574-6968
- Publication type
Journal Article
- DOI
10.1111/j.1574-6968.2009.01809.x