We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
FIS activates glnAp2 in Escherichia coli: role of a DNA bend centered at -55, upstream of the transcription start site.
- Authors
Huo, Yi-Xin; Nan, Bei-Yan; You, Cong-Hui; Tian, Zhe-Xian; Kolb, Annie; Wang, Yi-Ping
- Abstract
A binding site for the Escherichia coli nucleoid binding protein FIS (factor for inversion stimulation) was identified upstream of a sigma54-dependent promoter, glnAp2. The binding and bending center of FIS is positioned at -55 with respect to the transcription start site (+1). Binding of FIS at this site activates the transcription of glnAp2 both in vivo and in vitro. Furthermore, we substituted the FIS-mediated DNA bending with other protein (cAMP receptor protein or integration host factor)-mediated DNA bending, without changing the position of the bending center. In vitro transcription assays indicated that all DNA bends centered at -55 activate transcriptional initiation of glnAp2, especially when linear templates were used.
- Publication
FEMS microbiology letters, 2006, Vol 257, Issue 1, p99
- ISSN
0378-1097
- Publication type
Journal Article
- DOI
10.1111/j.1574-6968.2006.00150.x