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- Title
Tryptose phosphate broth improves Rickettsia felis replication in mammalian cells.
- Authors
Saisongkorh, Watcharee; El Karkouri, Khalid; Patrice, Jean-Yves; Bernard, Annick; Rolain, Jean-Marc; Raoult, Didier
- Abstract
In cell culture, Rickettsia felis grows only at low temperatures (< 31 °C). Therefore, its ability to enter, survive and grow in cell lines has primarily been tested in cells derived from amphibians and arthropods, which naturally grow at low temperatures, and only infrequently in mammalian cells. We subcultured R. felis in mammalian cells for more than 10 passages using media supplemented with tryptose phosphate broth (TPB) and found that TPB is critical for optimal growth of R. felis in mammalian cells.
- Publication
FEMS immunology and medical microbiology, 2012, Vol 64, Issue 1, p111
- ISSN
1574-695X
- Publication type
Journal Article
- DOI
10.1111/j.1574-695X.2011.00882.x