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- Title
Concordance Study Between the AmpFℓSTR<sup>®</sup> MiniFiler<sup>TM</sup> PCR Amplification Kit and Conventional STR Typing Kits.
- Authors
Hill, Carolyn R.; Kline, Margaret C.; Mulero, Julio J.; Lagacé, Robert E.; Chang, Chien‐Wei; Hennessy, Lori K.; Butler, John M.
- Abstract
The AmpFℓSTR® MiniFilerTM polymerase chain reaction amplification kit developed by Applied Biosystems enables size reduction on eight of the larger STR loci amplified in the Identifiler® kit, which will aid recovery of information from highly degraded DNA samples. The MiniFilerTM Kit amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1308 samples were evaluated with both the MiniFilerTM and Identifiler® STR kits: 449 African American, 445 Caucasian, 207 Hispanic, and 207 Asian individuals. Full concordance between Identifiler and MiniFiler Kits was observed in 99.7% (10,437 out of 10,464) STR allele calls compared. The 27 differences seen are listed in Table 1 and encompass the loci D13S317 ( n = 14) and D16S539 ( n = 10) as well as D18S51 ( n = 1), D7S820 ( n = 1), and CSF1PO ( n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex® 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift.
- Publication
Journal of Forensic Sciences, 2007, Vol 52, Issue 4, p870
- ISSN
0022-1198
- Publication type
Academic Journal
- DOI
10.1111/j.1556-4029.2007.00491.x