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- Title
Effects of morphine on gene expression in the rat amygdala.
- Authors
Parkitna, J. M. Rodriguez; Bilecki, W.; Mierzejewski, P.; Stefanski, R.; Ligeza, A.; Bargiela, A.; Ziolkowska, B.; Kostowski, W.; Przewlocki, R.
- Abstract
Influence of morphine self-administration on gene expression in the rat amygdala was studied using rat genome DNA arrays U34A from Affymetrix. Animals were trained to self-administer morphine, each having two‘yoked’ control animals, receiving passive injections of either morphine or saline. After 40 sessions of self-administration, amygdalae were removed, total RNA was isolated and used to prepare probes for Genechip® arrays. The treatment was found to significantly change abundance of 29 transcripts. Analysis by means of reverse transcription real-time PCR showed significant changes in abundance of five transcripts:γ protein kinase C (PKC), upstream binding factor 2 (UBF2), lysozyme, noggin and heat shock protein 70 (hsp70). After 30 days of forced abstinence from morphine self-administration, abundance of hsp70 and lysozyme returned to basal levels. Changes in abundance of UBF2 persisted, and abundance of three additional genes, namely nuclear factorI/A,γ1 subunit of GABAA receptor and the neuronal calcium sensor 1, changed. Additionally, acute as well as chronic intraperitoneal morphine administration changed the abundance of PKCγ,γ1 subunit of GABAA and hsp70 genes.
- Publication
Journal of Neurochemistry, 2004, Vol 91, Issue 1, p38
- ISSN
0022-3042
- Publication type
Academic Journal
- DOI
10.1111/j.1471-4159.2004.02697.x