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- Title
Detection of Burkholderia cepacia DNA from artificially infected EDTA-blood and lung tissue comparing different DNA isolation methods.
- Authors
Merk, S; Meyer, H; Greiser-Wilke, I; Sprague, L D; Neubauer, H
- Abstract
Bacterial DNA (Burkholderia cepacia) was prepared from artificially infected equine ethylenediaminetetraacetic acid (EDTA)-blood and lung tissue by using four standard methods (lysis buffer containing proteinase K, phenol/chloroform/isoamylalcohol-extraction, microwave-treatment, heat treatment) and six commercially available kits (Puregene, High Pure PCR Template Preparation Kit, InstaGene, QiaAmp Tissue Kit, DNAzol and Elu-Quik). After a subsequent polymerase chain reaction (PCR), their efficacy and sensitivity were compared. Concerning the detection limits, the simple lysis with a proteinase K-containing buffer led to the best results for EDTA-blood as well as for artificially infected lung tissue.
- Publication
Journal of veterinary medicine. B, Infectious diseases and veterinary public health, 2006, Vol 53, Issue 6, p281
- ISSN
0931-1793
- Publication type
Journal Article
- DOI
10.1111/j.1439-0450.2006.00956.x