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- Title
A Candida albicans homolog of CDC25 is functional in Saccharomyces cerevisiae.
- Authors
Goldberg, D; Marbach, I; Gross, E; Levitzki, A; Simchen, G
- Abstract
We have cloned, by functional complementation of the cdc25-2 mutation of Saccharomyces cerevisiae, a homolog of CDC25 from the pathogenic yeast Candida albicans. The new gene, named CSC25 codes for a 1333-amino-acid protein. The full length gene, as well as a truncated form coding for 795 amino acids, suppresses the thermosensitive phenotype of cdc25ts mutants. Biochemical analysis has shown that Csc25 activates the Ras/adenylyl cyclase pathway in S. cerevisiae at a rate two to three times faster than Cdc25, under the same conditions. The C-terminal domain of Csc25 is highly similar to the C-terminal domain of Cdc25, to almost the same extent as the C-terminus of the endogenous Cdc25 homolog Sdc25. We show that polyclonal anti-Cdc25 antibodies interact with Csc25 expressed in S. cerevisiae. In addition to the full length protein (approximately 150 kDa), we have found a approximately 50-kDa polypeptide which seems to include the C-terminus of the CSC25 gene product.
- Publication
European journal of biochemistry, 1993, Vol 213, Issue 1, p195
- ISSN
0014-2956
- Publication type
Journal Article
- DOI
10.1111/j.1432-1033.1993.tb17748.x