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- Title
The EcoDXX1 restriction and modification system of Escherichia coli ET7. Purification, subunit structure and properties of the restriction endonuclease.
- Authors
Piekarowicz, A; Goguen, J D; Skrzypek, E
- Abstract
The Escherichia coli plasmid pDXX1 codes for a new restriction-modification system. The specific restriction endonuclease coded by this system has been purified by a procedure that includes phosphocellulose and heparin-agarose chromatography. Sedimentation on glycerol gradients showed one peak of activity with a value of about 12 S. The highly purified enzyme require ATP and Mg2+ for activity as well as S-adenosylmethionine, although some S-adenosylmethionine molecules are probably bound to the enzyme. The enzyme does not cleave lambda DNA at well-defined sites and has a strong non-modified DNA-dependent ATPase activity. The enzyme has also methylase activity acting against non-modified DNA.
- Publication
European journal of biochemistry, 1985, Vol 152, Issue 2, p387
- ISSN
0014-2956
- Publication type
Journal Article
- DOI
10.1111/j.1432-1033.1985.tb09209.x