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- Title
Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets.
- Authors
de Lillo, A; Ashley, F P; Palmer, R M; Munson, M A; Kyriacou, L; Weightman, A J; Wade, W G
- Abstract
Molecular ecological analysis based on 16S rRNA gene sequence analysis is well established for the characterisation of complex bacterial communities. However, 'universal' PCR primers can introduce biases into the analysis of the species composition of clone libraries because of mismatches between the primer and target organism sequences. In this study, three universal primer sets were compared for the analysis of the microflora in subgingival plaque.
- Publication
Oral microbiology and immunology, 2006, Vol 21, Issue 1, p61
- ISSN
0902-0055
- Publication type
Journal Article
- DOI
10.1111/j.1399-302X.2005.00255.x