We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Arabidopsis XXT5 gene encodes a putative alpha-1,6-xylosyltransferase that is involved in xyloglucan biosynthesis.
- Authors
Zabotina, Olga A; van de Ven, Wilhelmina T G; Freshour, Glenn; Drakakaki, Georgia; Cavalier, David; Mouille, Gregory; Hahn, Michael G; Keegstra, Kenneth; Raikhel, Natasha V
- Abstract
The function of a putative xyloglucan xylosyltransferase from Arabidopsis thaliana (At1g74380; XXT5) was studied. The XXT5 gene is expressed in all plant tissues, with higher levels of expression in roots, stems and cauline leaves. A T-DNA insertion in the XXT5 gene generates a readily visible root hair phenotype (root hairs are shorter and form bubble-like extrusions at the tip), and also causes the alteration of the main root cellular morphology. Biochemical characterization of cell wall polysaccharides isolated from xxt5 mutant seedlings demonstrated decreased xyloglucan quantity and reduced glucan backbone substitution with xylosyl residues. Immunohistochemical analyses of xxt5 plants revealed a selective decrease in some xyloglucan epitopes, whereas the distribution patterns of epitopes characteristic for other cell wall polysaccharides remained undisturbed. Transformation of xxt5 plants with a 35S::HA-XXT5 construct resulted in complementation of the morphological, biochemical and immunological phenotypes, restoring xyloglucan content and composition to wild-type levels. These data provide evidence that XXT5 is a xyloglucan alpha-1,6-xylosyltransferase, and functions in the biosynthesis of xyloglucan.
- Publication
The Plant journal : for cell and molecular biology, 2008, Vol 56, Issue 1, p101
- ISSN
1365-313X
- Publication type
Journal Article
- DOI
10.1111/j.1365-313X.2008.03580.x