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- Title
EGY1encodes a membrane-associated and ATP-independent metalloprotease that is required for chloroplast development.
- Authors
Gu Chen; Yu Rong Bi; Ning Li
- Abstract
Chloroplast development requires coordinated expression of both nuclear- and chloroplast-encoded genes. To better understand the roles played by nuclear-encoded chloroplast proteins in chloroplast biogenesis, we isolated an Arabidopsis mutant,egy1-1, which has a dual phenotype, reduced chlorophyll accumulation and abnormal hypocotyl gravicurvature. Subsequent map-based cloning and DNA sequencing of the mutant gene revealed a 10-bp deletion in anEGY1gene, which encodes a 59-kDa metalloprotease that contains eighttrans-membrane domains at its C-terminus, and carries outβ-casein degradation in an ATP-independent manner. EGY1 protein accumulation varies between tissue types, being most prominent in leaf and stem tissues, and is responsive to light and ethylene. EGY1-GFP hybrid proteins are localized in the chloroplast.egy1mutant chloroplasts had reduced granal thylakoids and poorly developed lamellae networks. Furthermore, the accumulation of chlorophyll a/b binding proteins of the light-harvesting complexes I and II (Lhca and Lhcb) are significantly decreased in three separate loss-of-functionegy1mutants. Taken together, these results suggest that EGY1 metalloprotease is required for chloroplast development and, hence, a defectiveEGY1gene has pleiotropic effects both on chloroplast development and on ethylene-dependent gravitropism of light-grown hypocotyls.
- Publication
Plant Journal, 2005, Vol 41, Issue 3, p364
- ISSN
0960-7412
- Publication type
Academic Journal
- DOI
10.1111/j.1365-313X.2004.02308.x