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- Title
An endocytic mechanism for haemoglobin-iron acquisition in Candida albicans.
- Authors
Weissman, Ziva; Shemer, Revital; Conibear, Elizabeth; Kornitzer, Daniel
- Abstract
The fungal pathogen Candida albicans is able to utilize haemin and haemoglobin as iron sources. Haem-iron utilization is facilitated by Rbt5, an extracellular, glycosylphophatidylinositol (GPI)-anchored, haemin- and haemoglobin-binding protein. Here, we show that Rbt5 and its close homologue Rbt51 are short-lived plasma membrane proteins, degradation of which depends on vacuolar activity. Rbt5 facilitates the rapid endocytosis of haemoglobin into the C. albicans vacuole. We relied on recapitulation of the Rbt51-dependent haem-iron utilization in Saccharomyces cerevisiae to identify mutants defective in haemoglobin utilization. Homologues of representative mutants in S. cerevisiae were deleted in C. albicans and tested for haemoglobin-iron utilization and haemoglobin uptake. These mutants define a novel endocytosis-mediated haemoglobin utilization mechanism that depends on acidification of the lumen of the late secretory pathway, on a type I myosin and on the activity of the ESCRT pathway.
- Publication
Molecular microbiology, 2008, Vol 69, Issue 1, p201
- ISSN
1365-2958
- Publication type
Journal Article
- DOI
10.1111/j.1365-2958.2008.06277.x