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- Title
A glutamate-alanine-leucine (EAL) domain protein of Salmonella controls bacterial survival in mice, antioxidant defence and killing of macrophages: role of cyclic diGMP.
- Authors
Hisert, Katherine B; MacCoss, Michael; Shiloh, Michael U; Darwin, K Heran; Singh, Shaneen; Jones, Roger A; Ehrt, Sabine; Zhang, Zhaoying; Gaffney, Barbara L; Gandotra, Sheetal; Holden, David W; Murray, Diana; Nathan, Carl
- Abstract
Signature-tagged transposon mutagenesis of Salmonella with differential recovery from wild-type and immunodeficient mice revealed that the gene here named cdgR[for c-diguanylate (c-diGMP) regulator] is required for the bacterium to resist host phagocyte oxidase in vivo. CdgR consists solely of a glutamate-alanine-leucine (EAL) domain, a predicted cyclic diGMP (c-diGMP) phosphodiesterase. Disruption of cdgR decreased bacterial resistance to hydrogen peroxide and accelerated bacterial killing of macrophages. An ultrasensitive assay revealed c-diGMP in wild-type Salmonella with increased levels in the CdgR-deficient mutant. Thus, besides its known role in regulating cellulose synthesis and biofilm formation, bacterial c-diGMP also regulates host-pathogen interactions involving antioxidant defence and cytotoxicity.
- Publication
Molecular microbiology, 2005, Vol 56, Issue 5, p1234
- ISSN
0950-382X
- Publication type
Journal Article
- DOI
10.1111/j.1365-2958.2005.04632.x