We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
mRNA degradation in Escherichia coli: a novel factor which impedes the exoribonucleolytic activity of PNPase at stem-loop structures.
- Authors
Causton, H; Py, B; McLaren, R S; Higgins, C F
- Abstract
Stem-loop structures can protect upstream mRNA from degradation by impeding the processive activities of 3'-5' exoribonucleases. The ability of such structures to impede exonuclease activity in vitro is insufficient to account for the stability they can confer on mRNA in vivo. In this study we identify a factor from Escherichia coli which specifically impedes the processive activity of the 3'-5' exonuclease PNPase at stem-loop structures in vitro. This factor can, potentially, reconcile the apparent discrepancy between the ability of 3' stem-loop structures to stabilize upstream mRNA in vitro and in vivo. Its mechanism of action, and possible role in regulating mRNA degradation, is discussed.
- Publication
Molecular microbiology, 1994, Vol 14, Issue 4, p731
- ISSN
0950-382X
- Publication type
Journal Article
- DOI
10.1111/j.1365-2958.1994.tb01310.x