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- Title
Iron, DtxR, and the regulation of diphtheria toxin expression.
- Authors
Xu Tao; Schiering, Nikolaus; Hui-yan Zeng; Ringe, Dagmar; Murphy, John R.
- Abstract
In recent years considerable advances have been made in the understanding of the molecular basis of iron-mediated regulation of diphtheria toxin expression. The <em>tox</em> gene has been shown to be regulated by the heavy metal ion-activated regulatory element DtxR. In the presence of divalent heavy metal ions, DtxR becomes activated and binds to a 9 bp interrupted palindromic sequence. The consensus-binding site has been determined by both the sequence analysis of DtxR-responsive operators cloned from genomic libraries of <em>Corynebacterium diphtheriae</em> as well as by <em>in vitro</em> genetic methods using cyclic amplification of selected targets (CASTing). It is now clear that DtxR functions as a global iron-sensitive regulatory element in the control of gene expression in <em>C. diphtheriae</em>. In addition, the metal ion-activation domain of DtxR is being characterized by both mutational analysis and determination of the X-ray structure at 3.0 A resolution.
- Publication
Molecular Microbiology, 1994, Vol 14, Issue 2, p191
- ISSN
0950-382X
- Publication type
Academic Journal
- DOI
10.1111/j.1365-2958.1994.tb01280.x