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- Title
Synthesis of ribosomal proteins during growth of <em>Streptomyces coelicolor</em>.
- Abstract
Changes in expression of ribosomal protein genes during growth and stationary phase of <em>Streptomyces coelicolor</em> A3(2) in liquid medium were studied. Proteins being synthesized were pulse-labelled with [35]-methionine, separated by two-dimensional poly-acrylamide gel electrophoresis, and quantified using the Biolmage computer software. Most of the ribosomal proteins were synthesized throughout the life cycle. Exceptions were two proteins whose synthesis drastically decreased at the approach of stationary phase. These two proteins were identified in purified ribosomes as homologues of <em>Escherichia coli</em> ribosomal proteins L10 and L7/L12, using antibodies raised against fusion proteins between these ribosomal proteins and <em>Escherichia coli</em> β-galactosidase. The genes (<em>rpIJ</em> and <em>rpIL</em>) encoding the L10 and L7/L12 proteins were contained in a 1.2 kb <em>BamHI</em> fragment that was cloned and sequenced. The linkage and order of the genes coincide with other L10-L7/L12 operons. However, L11 and L1 genes were not present immediately upstream of the L10 gene, as is the case for <em>E. coli</em> and other bacteria, instead, two open reading frames of unknown function were found immediately upstream of the L10 gene, in an adjacent 1.9 kb <em>BamHI</em> fragment.
- Publication
Molecular Microbiology, 1994, Vol 12, Issue 3, p375
- ISSN
0950-382X
- Publication type
Academic Journal
- DOI
10.1111/j.1365-2958.1994.tb01027.x