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- Title
Enhancing collection efficiency in large field of view multiphoton microscopy.
- Authors
McMullen, J D; Kwan, A C; Williams, R M; Zipfel, W R
- Abstract
Many multiphoton imaging applications would benefit from a larger field of view; however, large field of views (>mm) require low magnification objectives which have low light collection efficiencies. We demonstrate a light collection system mounted on a low magnification objective that increases fluorescence collection by as much as 20-fold in scattering tissues. This peripheral detector results in an effective numerical aperture of collection >0.8 with a 3-4 mm field of view.
- Publication
Journal of microscopy, 2011, Vol 241, Issue 2, p119
- ISSN
1365-2818
- Publication type
Journal Article
- DOI
10.1111/j.1365-2818.2010.03419.x