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- Title
Modelling the application of adaptive optics to wide-field microscope live imaging.
- Authors
Kam, Zvi; Kner, Peter; Agard, David; Sedat, John W
- Abstract
Wide-field fluorescence microscopy is an essential tool in modern cell biology. Unfortunately the image quality of fluorescence microscopes is often significantly degraded due to aberrations that occur under normal imaging conditions. In this article, we examine the use of adaptive optics technology to dynamically correct these problems to achieve close to ideal diffraction limited performance. Simultaneously, this technology also allows ultra-rapid focusing without having to move either the stage or the objective lens. We perform optical simulations to demonstrate the degree of correction that can be achieved.
- Publication
Journal of microscopy, 2007, Vol 226, Issue Pt 1, p33
- ISSN
0022-2720
- Publication type
Journal Article
- DOI
10.1111/j.1365-2818.2007.01751.x