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- Title
SHORT COMMUNICATION Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data.
- Authors
Clayton, A.H.A.; Hanley, Q.S.; Ver Veer, P.J.
- Abstract
Graphical representation of fluorescence lifetime imaging microscopy data demonstrates that a mixture of two components with single exponential decays can be resolved by single frequency measurements. We derive a method based on linear fitting that allows the calculation of the fluorescence lifetimes of the two components. We show that introduction of proper error-weighting results in a non-linear method that is mathematically identical to a global analysis algorithm that was recently derived. The graphical approach was applied to cellular data obtained from a lifetime-based phosphorylation assay for the epidermal growth factor receptor and yielded results similar to those obtained by a global analysis algorithm.
- Publication
Journal of Microscopy, 2004, Vol 213, Issue 1, p1
- ISSN
0022-2720
- Publication type
Academic Journal
- DOI
10.1111/j.1365-2818.2004.01265.x