We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cloning and molecular characterization of two invertebrate-type lysozymes from Anopheles gambiae.
- Authors
Paskewitz, S M; Li, B; Kajla, M K
- Abstract
We sequenced and characterized two novel invertebrate-type lysozymes from the mosquito Anopheles gambiae. Alignment and phylogenetic analysis of these and a number of related insect proteins identified through bioinformatics strategies showed a high degree of conservation of this protein family throughout the Class Insecta. Expression profiles were examined for the two mosquito genes through semiquantitative and real-time PCR analysis. Lys i-1 transcripts were found in adult females in the fat body and Malpighian tubules, whereas Lys i-2 was detected only in fat bodies. Blood-feeding resulted in significantly increased transcript abundance for both genes in the midguts. Neither gene was upregulated following bacterial challenge.
- Publication
Insect molecular biology, 2008, Vol 17, Issue 3, p217
- ISSN
1365-2583
- Publication type
Journal Article
- DOI
10.1111/j.1365-2583.2008.00797.x