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- Title
Computer-assisted pathological immunohistochemistry scoring is more time-effective than conventional scoring, but provides no analytical advantage.
- Authors
Chee Wee Ong; Lay Gek Kim; Hui Hui Kong; Lai Yee Low; Ting Ting Wang; Supriya, Srivastava; Kathiresan, Manickam; Richie Soong; Salto-Tellez, Manuel
- Abstract
Ong C W, Kim L G, Kong H H, Low L Y, Wang T T, Supriya S, Kathiresan M, Soong R & Salto-Tellez M (2010) Histopathology 56, 523–529 Computer-assisted pathological immunohistochemistry scoring is more time-effective than conventional scoring, but provides no analytical advantage Aims: Interpretation of immunohistochemistry is primarily done through human visual scoring while computer-assisted scoring is relatively uncommon. This study aimed to examine (i) the level of agreement between human visual and computer-assisted pathological scoring of immunoreactivity expression in colorectal cancers, (ii) whether computer-assisted scoring affects the prognostic significance of biomarkers, and (iii) whether computer-assisted pathological scoring provides any time-saving or reproducibility advantages. Methods and results: Tissue microarray blocks were constructed from the primary colorectal adenocarcinoma specimens of 486 patients. Scoring of the six markers [cytokeratin (CK) 7, CK20, cyclooxygenase-2, Ki67, p27 and p53] was done independently by a qualified pathologist, a trained scientist and the Ariol SL-50 (Applied Imaging). Univariate analysis showed that human visual and computer-assisted scoring were strongly correlated (all κ values >0.8). Both human visual and computer-assisted pathological scoring identified the same set of biomarkers with significant association with survival. Computer-assisted pathological scoring was shown to be a time-effective means of scoring larger numbers of slides (for high-throughput studies). Conclusions: Our results suggest that computer-assisted pathological scoring can be a viable alternative to pathologist scoring in a manner that is more practical and time-effective, but, interestingly, providing no analytical advantage.
- Publication
Histopathology, 2010, Vol 56, Issue 4, p523
- ISSN
0309-0167
- Publication type
Academic Journal
- DOI
10.1111/j.1365-2559.2010.03496.x