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- Title
Purification of FANCD2 sub-complexes.
- Authors
Gang Zhi; Xiaoyong Chen; Newcomb, William; Brown, Jay; Semmes, Oliver J.; Kupfer, Gary M.
- Abstract
Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.
- Publication
British Journal of Haematology, 2010, Vol 150, Issue 1, p88
- ISSN
0007-1048
- Publication type
Academic Journal
- DOI
10.1111/j.1365-2141.2010.08217.x