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- Title
Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine Sulfate) in the process of vaccine production.
- Authors
Sakoda, Yoshihiro; Okamatsu, Masatoshi; Isoda, Norikazu; Yamamoto, Naoki; Ozaki, Koichi; Umeda, Yasuto; Aoyama, Shigeyuki; Kida, Hiroshi
- Abstract
Affinity chromatography using sulfated, spherical cellulose beads (Cellufine Sulfate) was assessed for purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful for removing chicken egg-derived impurities from allantoic fluids containing influenza viruses; the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that use of Cellufine Sulfate is a practical method for primary purification of influenza viruses in the process of influenza vaccine production.
- Publication
Microbiology and immunology, 2012, Vol 56, Issue 7, p490
- ISSN
1348-0421
- Publication type
Journal Article
- DOI
10.1111/j.1348-0421.2012.00468.x