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- Title
Simulation of specimen-induced aberrations for objects with spherical and cylindrical symmetry.
- Authors
Schwertner, M; Booth, M J; Wilson, T
- Abstract
Wavefront aberrations caused by the refractive index structure of the specimen are known to compromise signal intensity and three-dimensional resolution in confocal and multiphoton microscopy. However, adaptive optics can measure and correct specimen-induced aberrations. For the design of an adaptive optics system, information on the type and amount of the aberration is required. We have previously described an interferometric set-up capable of measuring specimen-induced aberrations and a method for the extraction of the Zernike mode content. In this paper we have modelled specimen-induced aberrations caused by spherical and cylindrical objects using a ray tracing method. The Zernike mode content of the wavefronts was then extracted from the simulated wavefronts and compared with experimental results. Aberrations for a simple model of an oocyte cell consisting of two spherical regions and for a model of a well-characterized optical fibre are calculated. This simple model gave Zernike mode data that are in good agreement with experimental results.
- Publication
Journal of microscopy, 2004, Vol 215, Issue Pt 3, p271
- ISSN
0022-2720
- Publication type
Journal Article
- DOI
10.1111/j.0022-2720.2004.01371.x