We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification and characterization of two isozymic forms of arylamine N-acetyltransferase in Syrian hamster skin.
- Authors
Gaudet, S J; Slominski, A; Etminan, M; Pruski, D; Paus, R; Namboodiri, M A
- Abstract
Arylamine N-acetyltransferase (EC 2.3.1.5) activity was examined using skin from Syrian hamster. Two isozymes of arylamine N-acetyltransferase, designated NAT-1 and NAT-2, were detected on anion-exchange high-performance liquid chromatography analysis. Both enzyme activities had indistinguishable molecular masses (30 kDa), but differed significantly in their specificity toward the aromatic amines including serotonin, dopamine, methoxytryptamine, tryptamine, para-phenetidine, para-aminobenzoic acid, and sulphamethazine. Specifically, NAT-2 but not NAT-1 catalyzed acetylation of dopamine to N-acetyldopamine and acetylation of serotonin to form N-acetylserotonin, a direct precursor of melatonin. The two isozymes were also distinguishable based upon their sensitivity toward methotrexate inhibition (50% inhibiting dose for NAT-1 = 380 microM; NAT-2 > 2 mM). The presence of these two activities in the skin raises new questions about the physiologic role of this enzyme in general and in the skin-specific functions in particular.
- Publication
The Journal of investigative dermatology, 1993, Vol 101, Issue 5, p660
- ISSN
0022-202X
- Publication type
Journal Article
- DOI
10.1111/1523-1747.ep12371672