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- Title
A quick solution: ab initio structure determination of a 19 kDa metalloproteinase using ACORN.
- Authors
McAuley, K E; Jia-Xing, Y; Dodson, E J; Lehmbeck, J; Østergaard, P R; Wilson, K S
- Abstract
A data set from the metalloproteinase deuterolysin was collected at atomic resolution (1.0 A) with synchrotron radiation. The high resolution allowed the structure to be solved with the new direct-methods program ACORN using the coordinates of the Zn atom as a starting point. The phases obtained from ACORN were of sufficient quality to allow automated building to be carried out in ARP/wARP. Minimal manual rebuilding of the model was required and the structure determination was completed using the maximum-likelihood refinement program REFMAC. The whole process, starting from the processed and merged data and ending with a refined model, required less than 6 h of computational time.
- Publication
Acta crystallographica. Section D, Biological crystallography, 2001, Vol 57, Issue Pt 11, p1571
- ISSN
0907-4449
- Publication type
Journal Article
- DOI
10.1107/s090744490101335x