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- Title
Expression, purification and crystallization of human tau-protein kinase I/glycogen synthase kinase-3beta.
- Authors
Aoki, M; Iwamoto-Sugai, M; Sugiura, I; Sasaki, C; Hasegawa, T; Okumura, C; Sugio, S; Kohno, T; Matsuzaki, T
- Abstract
Human tau-protein kinase I (TPK-I; also known as glycogen synthase kinase-3beta, GSK-3beta) is a serine/threonine protein kinase. Full-length TPK-I/GSK-3beta was expressed in Escherichia coli as a fusion protein with a 6xHis tag at the C-terminus and was crystallized using the hanging-drop vapour-diffusion method. Prismatic crystals of dimensions 0.4 x 0.2 x 0.1 mm were obtained using 12-15%(w/v) polyethylene glycol 6000 as a precipitant at 278 K. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 82.9, b = 86.1, c = 178.1 A measured at 100 K, diffract to 2.3 A resolution and seem to contain two enzyme molecules per asymmetric unit.
- Publication
Acta crystallographica. Section D, Biological crystallography, 2000, Vol 56, Issue Pt 11, p1464
- ISSN
0907-4449
- Publication type
Journal Article
- DOI
10.1107/s0907444900010386