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- Title
Crystallization and preliminary X-ray diffraction analysis of bacteriophage varphi12 packaging factor P7.
- Authors
Kainov, Denis E; Simonov, Vladimir; Bamford, Dennis H; Tuma, Roman; Gottlieb, Paul; Wei, Hui; Walsh, Martin A; Belrhali, Hassan; Merckel, Michael C
- Abstract
Bacteriophage varphi12 protein P7 is a structural component of the polymerase complex and ensures stable packaging of the genomic RNA. varphi12 P7 has been cloned, purified and crystallized. Crystals belong to space group P3(2)21, with unit-cell parameters a = 75.7, b = 75.7, c = 45.2 A, alpha = 90, beta = 90, gamma = 120 degrees , and diffract beyond 2.0 A. Multiple anomalous dispersion data have been collected from crystals of selenomethionylated P7. Mass spectroscopy showed proteolysis of the crystallized protein and a truncated form, P7DeltaC, gave crystals of similar morphology. Cross-linking experiments implicated the N-terminal domain of P7 as being essential for dimerization.
- Publication
Acta crystallographica. Section D, Biological crystallography, 2004, Vol 60, Issue Pt 12 Pt 2, p2368
- ISSN
0907-4449
- Publication type
Journal Article
- DOI
10.1107/S0907444904026952