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- Title
Aqp 9 and brain tumour stem cells.
- Authors
Fossdal, Guri; Vik-Mo, Einar O; Sandberg, Cecilie; Varghese, Mercy; Kaarbø, Mari; Telmo, Emily; Langmoen, Iver A; Murrell, Wayne
- Abstract
Several studies have implicated the aquaporins (aqp) 1, 4, and 9 in the pathogenesis of malignant brain tumours, suggesting that they contribute to motility, invasiveness, and oedema formation and facilitate metabolism in tumour cells under hypoxic conditions. We have studied the expression of aqp1, 4, and 9 in biopsies from glioblastomas, isolated tumour stem cells grown in a tumoursphere assay and analyzed the progenitor and differentiated cells from these cultures. We have compared these to the situation in normal rat brain, its stem cells, and differentiated cells derived thereof. In short, qPCR in tumour tissue showed presence of aqp1, 4, and 9. In the tumour progenitor population, aqp9 was markedly more highly expressed, whilst in tumour-derived differentiated cells, aqp4 was downregulated. However, immunostaining did not reveal increased protein expression of aqp9 in the tumourspheres containing progenitor cells; in contrast, its expression (both mRNA and protein) was high in differentiated cultures. We, therefore, propose that aquaporin 9 may have a central role in the tumorigenesis of glioblastoma.
- Publication
TheScientificWorldJournal, 2012, Vol 2012, p915176
- ISSN
1537-744X
- Publication type
Journal Article
- DOI
10.1100/2012/915176