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- Title
Investigation of the role of IL-1 and TNF in matrix degradation in the intervertebral disc.
- Authors
J. A. Hoyland; C. Le Maitre; A. J. Freemont
- Abstract
Objective. To establish if IL-1 or TNF regulates matrix degradation in the non-degenerate or degenerate intervertebral disc (IVD). Methods. In situ zymography (ISZ) has been used to investigate the role of IL-1 and TNF in the matrix degradation characterizing symptomatic IVD degeneration. ISZ employed three substrates (gelatin, collagen II, casein) and four different challenges, IL-1β, IL-1 receptor antagonist (IL-1Ra), TNF-α and anti-TNF. Results. We have shown for the first time that whilst IL-1β will stimulate and IL-1 receptor antagonist will inhibit matrix degradation in intact human IVD tissue, neither TNF-α nor anti-TNF have any measurable effect on degradation of these matrices. Conclusion. This study has addressed a current area of controversy in IVD biology, namely, whether either IL-1 or TNF or both are involved in driving matrix degradation. Our data indicate that IL-1 is a key cytokine mediating matrix degradation in the IVD and therefore a therapeutic target.
- Publication
Rheumatology, 2008, Vol 47, Issue 6, p809
- ISSN
1462-0324
- Publication type
Academic Journal
- DOI
10.1093/rheumatology/ken056