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- Title
Secretion-and-capture cell-surface display for selection of target-binding proteins.
- Authors
Rakestraw, J A; Aird, D; Aha, P M; Baynes, B M; Lipovsek, D
- Abstract
This report describes a new cell-surface display system, the Secretion and Capture Technology (SECANT™) platform, which relies on in vivo biotinylation of the protein of interest followed by its capture on the avidinated surface of the parent cell. Cell sorting techniques are then used to isolate clones that display target-binding protein. A distinguishing feature of this method is its ability to display complex proteins, such as full-length immunoglobulin G (IgG) antibodies, on living cells. In this proof-of-concept study, Saccharomyces cerevisiae cells that displayed Herceptin IgG were isolated from a 10,000-fold excess of cells that displayed a lysozyme-binding antibody.
- Publication
Protein engineering, design & selection : PEDS, 2011, Vol 24, Issue 6, p525
- ISSN
1741-0134
- Publication type
Journal Article
- DOI
10.1093/protein/gzr008