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- Title
2-Hydroxyisoflavanone Dehydratase is a Critical Determinant of Isoflavone Productivity in Hairy Root Cultures of Lotus japonicus.
- Authors
Masayuki Shimamura; Tomoyoshi Akashi; Nozomu Sakurai; Hideyuki Suzuki; Kazuki Saito; Daisuke Shibata; Shin-ichi Ayabe; Toshio Aoki
- Abstract
Hairy root cultures of a model legume, Lotus japonicus, were established to characterize two heterologous cDNAs encoding enzymes involved in isoflavone biosynthesis, i.e. licorice 2-hydroxyisoflavanone synthase (IFS) and soybean 2-hydroxyisoflavanone dehydratase (HID) catalyzing sequential reactions to yield isoflavones. While the control and the IFS overexpressor did not accumulate detectable isoflavones, the HID overexpressors did accumulate daidzein and genistein, showing that HID is a critical determinant of isoflavone productivity. Production of coumestrol in all the genotypes and isoliquiritigenin/liquiritigenin in IFS + HID-overexpressing lines was also noted. These results provide insight into the regulatory mechanism that controls isoflavonoid biosynthesis.
- Publication
Plant & Cell Physiology, 2007, Vol 48, Issue 11, p1652
- ISSN
0032-0781
- Publication type
Academic Journal
- DOI
10.1093/pcp/pcm125